HPLC RADIOACTIVITY MONITORS
Model # SSS-33
FEATURES:
- HIGH SENSITIVITY H-3, C-14, ALL BETA EMITTERS AND LOW
ENERGY GAMMA EMITTERS
- DUAL PM TUBE DESIGN
- WIDE CHOICE OF FLOW CELLS TO FIT ALL APPLICATIONS
- SOPHISTICATED COMPUTER DATA ANALYSES (OPTIONAL)
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Figure 1: SSS-33
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APPLICATION: The SSS-33 Radioactivity
Monitors accurately quantitatively measure Carbon-14, Tritium and
other radioactive labels used in conjunction with liquid chromatography
and HPLC. These fine measurement systems are of particular interest
to researchers working in fields of metabolism, toxicology, pesticides,
agriculture, pharmacology, nuclear medicine, and other bio-chemistry
areas. The SSS-33 Flow cell counting system allows high sensitivity
and quantitative measurement of any Radioisotope labeled fluid stream
from HPLC, LC or other source.
Single, dual or triple labeled samples can be measured.
SYSTEM DESCRIPTION:
Measuring Principle: The most sensitive
method of detecting and quantitating beta emitting isotopes is to
intimately mix the effluent stream with liquid or granular scintillator
and count each individual scintillation event with a dual photomultiplier
coincidence counter. Then the signal is further selected by an energy
analyzer and this true signal is taken as a function of time. Sophisticated
computer data analysis and hard copy print out are provided.
Sample Stream Flow Path: sample stream from HPLC, LC or other
source -
(a) is continuously mixed with precisely measured amount of liquid scintillation fluor, or,
(b) flows directly into packed detection flow cell.
Detection flow cell is optically coupled to two selected photo-multiplier
tubes which count in coincidence mode. Coincidence counting eliminates
photo-cathode noise pulses that are always present in any one PM tube
system. Extremely good sensitivities and figure of merit for low energy
betas such as H-3 and C-14 are thus achieved.
Alternatively, in case (b) above, a ``packed'' flow cell
is utilized. That is, the flow cell is packed with small scintillation
crystals, like fine sand. The sample flows through this bed of chemically
inert crystals. Scintillation counting occurs, and the sample stream
emerges pristine, i.e., no scintillation fluor dissolves in the sample
stream.
Flow cells are available packed with CaFl crystals or anthracene
for aqueous solutions, glass scintillation crystals for corrosive
solutions, or plastic scintillators for amino acid analysis.
Gamma ray counting is achieved by inserting and optically
coupling a NaI(Tl) scintillation crystal between the flow cell and
one or both of the PM tubes. (Coincidence counting is not used for
gammas.) Other flow cell types are available on request.
The various fractions in the sample stream can be collected
in a fraction collector, or the sample can be further measured or
purified, if desired.
Optional peak detection and flow time delay features allow
peak(s) to be collected while waste liquid flows into separate container.
TA TECHNICAL
ASSOCIATES
7051 ETON AVENUE * CANOGA PARK, CA 91303
TELEPHONE
(818) 883-7043 * FAX(818) 883-6103
e-mail:
tagold@nwc.net www.tech-associates.com
HPLC RADIOACTIVITY
MONITORS
Model # SSS-33
DATA ANALYSIS AND PRESENTATION:
Scintillation counts which are detected simultaneously
by both PM tubes are processed by a fully adjustable single channel
analyzer which is centered on the energy peak of the isotope being
measured. This deletes both higher energy pulses from background radiation
and lower energy counts from the PM tube or circuit noise. The pulses
are then fed to a counter and then to a computer, printer, 8'' chart
recorder and/or standard chromatography integrator. (This allows long
count times for measurement of very minute samples as well as completely
elimination artifacts caused by ratemeter time constants such as tailing
or spreading of peaks.)
Optional Interfaces and Outputs:
- Integrator Interface (Analogue System) Module #
MPL-5AV for input into all chromatography
integrators and data collection/analysis systems.
- TTL Pulse output (all systems)
- Computer Interface RS-232 serial output, Module
# MCC-23. Complete plug compatibility
with the standard input for computer.
Computer Integration and Analysis with additional
Hard Copy Readout:
Data Processor
(The following specifications are based on use of
TA's SSS-33 with independent chromatography system. SSS-33 can drive
any chromatography integrator analyzer.)
- Internal memory for up to 6 hours general chromatogram
storage.
- BASIC programming capability is standard.
- Full alphanumeric capability.
Data processor provides all data processing functions
required in chromatography.
Only three minutes needed for reprocessing of one-hour
chromatogram.
- One chromatogram can be reprocessed any number of
times using different parameters.
- Long term chromatogram (e.g. 6 hours at 0.5 second
sampling) can be accurately stored. Several different chromatograms
can be stored.
Graphics, such as calibration curves and bar graphs,
can be displayed and hard-copied on printer/plotter by simple keyboard
command.
Data Analysis and Read-Out Options:
- Data goes directly to computer by RS-232 interface
to be analyzed and displayed by chromatography peak analysis software
available from TA or use customer's software package.
- Data goes to stand-alone chromatography integrator
from HP, Nelson, Shimadzu, etc...
- Raw Data printed on digital printer.
- Raw Data drives 8'' analog chart recorder.
For each of these items: PC, PC software, integrator,
printer, or chart recorder can be provided either by Technical Associates
or the customer can provide their own.
TA TECHNICAL
ASSOCIATES
7051 ETON AVENUE * CANOGA PARK, CA 91303
TELEPHONE
(818) 883-7043 * FAX(818) 883-6103
e-mail:
tagold@nwc.net www.tech-associates.com
HPLC RADIOACTIVITY MONITORS
Model # SSS-33
TA TECHNICAL
ASSOCIATES
7051 ETON AVENUE * CANOGA PARK, CA 91303
TELEPHONE
(818) 883-7043 * FAX(818) 883-6103
e-mail:
tagold@nwc.net www.tech-associates.com
HPLC RADIOACTIVITY MONITORS
Model # SSS-33
TA TECHNICAL
ASSOCIATES
7051 ETON AVENUE * CANOGA PARK, CA 91303
TELEPHONE
(818) 883-7043 * FAX(818) 883-6103
e-mail:
tagold@nwc.net www.tech-associates.com